Objective To investigate the effect of autologous concentrated growth factor (CGF) on hair loss during the shedding phase in patients after hair transplantation. Methods A total of 80 patients who underwent hair transplantation at the Department of Dermatology and Venereology, China-Japan Friendship Hospital from May 2023 to December 2024 were selected as the research subjects. According to the random number table method, they were divided into the control group and the observation group, with 40 patients in each group. The control group received conventional intervention, and the observation group received local injection of CGF on the basis of the control group. The hair density, follicle survival rate, hair loss during the shedding phase, existing hair quantity and adverse reaction rate were compared between the two groups. Results The hair density and follicle survival rate in the observation group at 12 months after operation were higher than those in the control group (P<0.05). The number of hair loss in the observation group at 1, 2 and 3 months after operation was less than that in the control group (P<0.05). The number of existing hair in the observation group at 1, 2 and 3 months after operation was more than that in the control group (P<0.05). There was no signiffcant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion Local injection of CGF can effectively reduce the number of hair loss during shedding phase after hair transplantation, improve postoperative hair density and follicle survival rate, with good safety.