Objective To explore the application effect of gold microneedling combined with non-crosslinked hyaluronic acid and type Ⅲ collagen in periorbital rejuvenation. Methods A total of 70 patients who underwent periorbital rejuvenation treatment in Shanghai Yestar Medical Aesthetic Hospital from February to April 2024 were selected. According to the random number table method, they were divided into the control group and the study group, with 35 patients in each group. The control group was treated with non-crosslinked hyaluronic acid combined with type Ⅲ collagen, and the study group was treated with gold microneedling on the basis of the control group's regimen. The clinical efficacy, periorbital skin characteristics and Wrinkle Severity Rating Scale scores were compared between the two groups. Results The periorbital skin improvement rate of the study group (94.29%) was higher than that of the control group (77.14%) (P <0.05). After treatment, the scores of periorbital skin characteristics in the study group were all lower than those in the control group (P <0.05). The WSRS scores in the study group at 2 and 6 months after treatment were lower than those of the control group (P <0.05). Conclusion Gold microneedling combined with non-crosslinked hyaluronic acid and type Ⅲ collagen can effectively enhance periorbital skin elasticity, improve skin texture, fade fine lines, boost skin moisture and radiance, and elevate the overall aesthetic effect of periorbital area, which holds high application value in periorbital rejuvenation.