Objective To analyze the effect of glycolic acid combined with photorejuvenation on the pigmented spot area in patients with post-acne hyperpigmentation. Methods A total of 80 patients with post-acne hyperpigmentation admitted to the Department of Dermatology, the People's Hospital of Lincang from January 2022 to May 2024 were selected, and they were divided into the control group and the study group by the random number table method, with 40 patients in each group. The control group was treated with glycolic acid alone, and the study group was treated with glycolic acid combined with photorejuvenation. The pigmented spot area, treatment effect, skin visual effect, aesthetic effect and adverse reaction rate were compared between the two groups. Results The pigmented spot area of the two groups after treatment was smaller than that before treatment, and that of the study group was smaller than that of the control group (P <0.05). The total effective rate of the study group (97.50%) was higher than that of the control group (85.00%) (P <0.05). The skin visual effect score of the two groups after treatment was lower than that before treatment, and that of the study group was lower than that of the control group (P <0.05). The subjective and objective aesthetic scores of the two groups after treatment were higher than those before treatment, and those of the study group were higher than those of the control group (P<0.05). There was no statistically significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion Glycolic acid combined with photorejuvenation has a definite effect in the treatment of post-acne hyperpigmentation. It can effectively reduce the pigmented spot area, help to improve the skin visual effect and aesthetic effect, and does not increase the risk of adverse reactions, with high application safety.