Objective To analyze the effect of orthodontic treatment with micro-implant on dental aesthetics and oral function in patients with malocclusion. Methods A total of 80 patients with malocclusion admitted to the Department of Stomatology, Ningxia GEM Flower Hospital from January 2021 to January 2024 were selected as the research subjects. According to different treatment plans, they were divided into the reference group (n =39) and the study group (n =41). The reference group received traditional orthodontic treatment, and the study group received orthodontic treatment with micro-implant. The dental aesthetics, oral function, orthodontic treatment effect and complications were compared between the two groups. Results The score of dental aesthetics in the two groups after treatment was higher than that before treatment, and that in the study group was higher than that in the reference group (P <0.05). The masticatory efficiency and occlusal force in the two groups after treatment were higher than those before treatment, and those in the study group were higher than those in the reference group (P <0.05). The total effective rate of orthodontic treatment in the study group (95.12%) was higher than that in the reference group (74.36%) (P <0.05). The incidence of complications in the study group (2.44%) was lower than that in the reference group (20.51%) (P <0.05). Conclusion The application of orthodontic treatment with micro-implant in patients with malocclusion can effectively improve the dental aesthetics and oral function, which is beneficial to improving the orthodontic treatment effect and reducing the risk of complications. It is worthy of clinical application.